Fig 4. Specificity analysis of the 3F8 BiTE.

Binding of 3F8 to HPV-E7p11/HLA-A2 complex. T2 cells alone [A] or pulsed with HPV-E7p11-19 [B], or RMF peptide [C] at a concentration of 20ug/ml overnight. Cells were washed and stained with 3F8 BiTE [3ug/ml], followed by secondary antibody to His-tag. Binding of 3F8 BiTE to cell surface CD3. Jurkat cells [D] or primary human T cells [E] were stained with 3F8 or control BiTE at a concentration of 3ug/ml, followed by anti-His-PE secondary antibody. A control anti-CD3 antibody was used to confirm the CD3 expression.