Fig 5. Cytotoxicity of the 3F8 BiTE.

T2 cells alone, or pulsed with HPV-E7p11-19 or RMF control peptide at 50ug/ml were incubated with human PBMCs at E:T ratio of 20:1 and the 3F8 BiTE [Fig 5A], or control BiTE [Fig 5B] at indicated concentrations for 5 hrs and the cytotoxicity was measured by ⁵¹Cr-release assay. Similarly, 3F8 BiTE was tested for its cytotoxicity against CasKi [Fig 5C] without HPV E7-11 peptide pulsing at indicated concentrations. Each data point is the average of triplicate cultures and representative of three similar experiments.