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Histones do not cause activation and nuclear re-localization of NF-κB in HL-1 cardiomyocytes. HL-1 cells were treated with vehicle, 10 ng/mL TNFα or 10 μg/mL histones for 40 min, before fixation and staining of NF-κB. Only TNFα caused significant nuclear relocalization as shown in representative images and quantification (N = 3 experiments). Statistical analysis by one-way ANOVA with Tukey post-test. *=P < 0.0001; ns = non-significant, in comparison to vehicle.
