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Inhibition of autophagy attenuates ischemia-reperfusion injury in rat brains. (A) 2,3,5-Triphenyltetrazolium chloride staining and infarct volume; n=3. (B) Hematoxylin and eosin staining; n=3. (C and D) TUNEL assay was used to detect the level of apoptosis; n=3. (E and F) Western blot analysis of apoptosis-related proteins; n=6. ***P<0.001 vs. sham group; #P<0.05, ##P<0.01 and ###P<0.001 vs. model group. Sham, sham-operated group; 3-MA, 3-methyladenine.
