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. 2022 Mar 10;49(5):61. doi: 10.3892/ijmm.2022.5117

Figure 4.

Figure 4

High expression of Beclin-1 significantly attenuates oxygen-glucose deprivation/reperfusion-induced neuronal injury. (A) Reverse transcription-quantitative PCR was performed to evaluate the plasmid transfection efficiency. (B) CCK-8 assay. (C and D) Flow cytometry was performed to evaluate apoptosis. (E) Western blot analysis of cellular autophagy-associated proteins. ***P<0.001 vs. control; #P<0.05, ##P<0.01 and ###P<0.001 vs. model group; &P<0.05, &&P<0.01 and &&&P<0.001 vs. 3-MA + OE-NC group. 3-MA, methyladenine; OE-NC, overexpression-negative control; OE-BECN1, Beclin-1 overexpression vector.