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High expression of Beclin-1 leads to a rebound in autophagy in response to 3-MA inhibition to a certain extent. (A) Immunofluorescence staining for LC3 (red) and DAPI (blue) in the hippocampal areas of each group. (B) Western blot analysis of cellular autophagy-associated proteins. ***P<0.001 vs. control; ###P<0.001 vs. model group; &P<0.05 and &&&P<0.001 vs. 3-MA + OE-NC group. 3-MA, methyladenine; OE-NC, overexpression-negative control; OE-BECN1, Beclin-1 overexpression vector.
