Fig. 4.

Isothermal amplification in point-of-care testing (POCT). a Design and fabrication of the rotate and react SlipChip. After plasma bonding, a screw-nut suite was used to assemble the upper and lower chips to form the final chip (adapted from [76]). b Schematic illustration of the MI-IF-RPA system for COVID-19 detection (adapted from [85]). c Schematic of wearable RPA testing for rapid detection of HIV-1 DNA (adapted from [86]). SE Salmonella enterica, VF Vibrio fluvialis, VP Vibrio parahaemolyticus, BC Bacillus cereus, EC Escherichia coli, FAM carboxyfluorescein, HIV human immunodeficiency virus, RPA recombinase polymerase amplification, LED light emitting diode, MI-IF-RPA microfluidic-integrated lateral flow recombinase polymerase amplification