Fig. 7.

Typic digital nucleic acid assay platforms. a Workflow of rapid digital PCR method consists of four key steps: sample preparation, reaction mixture partition, amplification process, and targets quantification (adapted from [164]). b Schematic drawings demonstrate the droplet assay SlipChip for slip formation of droplets at high density (adapted from [71]). c Schematic of CARMEN-Cas13 workflow (adapted from [158]). d Overview of digitization-enhanced CRISPR/Cas-assisted one-pot virus detection (adapted from [169]). W/O water in oil, PDMS polydimethylsiloxane, PCR polymerase chain reaction, DAQ data acquisition, PID proportional integral derivative, CARMEN combinatorial arrayed reactions for multiplexed evaluation of nucleic acids, SARS-CoV-2 severe acute respiratory syndrome coronavirus 2, RT-RPA reverse-transcription recombinase polymerase amplification, S/B signal to background