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. 2022 Mar 10;2022:3058588. doi: 10.1155/2022/3058588

Figure 3.

Figure 3

Loss function of NPTX2 inhibits proliferation, migration, and immune escape of ccRCC cells. (a) mRNA and protein expression level in NPTX2-silenced ccRCC cell line. (b) Overexpression of miR-1251-5p suppressed expression of NPTX2 in protein level. (c) The association between miR-1251-5p and mutant category NPTX2 3′-UTR is depicted. (d) In cell lines cotransfected using wild/mutant type NPTX2 3′-UTR and miR-1251-5p copies, dual-luciferase tests were performed. (e) ccRCC cells of A498 and Caki-2 cells after knockdown of NPTX2 at 24 hours, 48 hours, and 72 hours are computed using MTT assay, respectively. (f) After knocking down NPTX2, the “colony formation assay” has been executed to compute cell's capability in A498 and Caki-2 cells. (g) “Transwell assay” is used to explore the potential of A498 and Caki-2 cells to migrate after knockdown of NPTX2 (100×). (h) An ELISA kit was used to assess the levels of IFN-α and TNF-γ in the supernatants of A498 and Caki-2 cells. ∗p < 0.05 and ∗∗p < 0.01.