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. 2022 Mar 10;2022:3058588. doi: 10.1155/2022/3058588

Figure 4.

Figure 4

Gain function of miR-1251-5p inhibits proliferation, migration, and immune escape of ccRCC cells. (a) The expression of miR-1251-5p in cell lines is identified using qRT-PCR. (b) MTT assay is used to determine cell's activity in A498 and Caki-2 cell lines after 24 h, 48 h, and 72 h. (c) The proliferation of A498 and Caki-2 cells is identified applying the colony formation test. (d) Transwell has been utilized to test the capability of A498 and Caki-2 cell lines to migrate. (e) An ELISA kit is used to assess the levels of IFN-α and TNF-γ in the supernatant of A498 and Caki-2 cells. ∗p < 0.05 and ∗∗p < 0.01.