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. 2022 Mar 10;2022:3058588. doi: 10.1155/2022/3058588

Figure 5.

Figure 5

NPTX2 can reverse the effects of miR-1251-5p overexpression. (a) The expression of miR-1251-5p and NPTX2 in transfected cells are identified using qRT-PCR. (b) A498 and Caki-2 cells after transfected with oe-NPTX2 and mimic-mir-1251-5p at 24 hours, 48 hours, and 72 hours are evaluated using MTT assay, correspondingly. (c) The viability of A498 and Caki-2 cells upon transaction with oe-NPTX2 and mimic-mir-1251-5p is determined using the colony formation assay. (d) The capacity of A498 and Caki-2 cells to migrate after being transfected with oe-NPTX2 and mimic-mir-1251-5p is tested using Transwell assay. (e) The levels of IFN-α and TNF-γ in the A498 and Caki-2 cell supernatant are estimated with an ELISA kit. ∗p < 0.05 and ∗∗p < 0.01.