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. 2022 Mar 10;2022:5687832. doi: 10.1155/2022/5687832

Figure 3.

Figure 3

Lico A inhibits c-Met signaling. (a) HCC827-GR (left) and PC-9-GR (right) cells were starved overnight with 0.1% FBS, and pretreated with Lico A for 4 h. Cells were stimulated with 20 ng HGF for 5 min, WCE was prepared and subjected to IB analysis. (b) HCC827-GR (left) and PC-9-GR (right) cells were treated with Lico A for 48 h, WCE was subjected to IB analysis. (c) qRT-PCR analyzes the mRNA levels of c-Met in Lico A-treated HCC827-GR (left) and PC-9-GR (right) cells. ∗p < 0.05 and ∗∗p < 0.01, (d) HCC827-GR (left) and PC-9-GR (right) cells were treated with Lico A for 48 h, followed by incubation with MG132 for 6 h, WCE was subjected to IB analysis. (e) HCC827-GR cells were treated with Lico A for 48 h, followed by incubation with MG132 for 6 h, WCE was subjected to immunoprecipitation (IP) and IB analysis.