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. 2022 Mar 10;2022:5687832. doi: 10.1155/2022/5687832

Figure 4.

Figure 4

c-Cbl is required for Lico A-induced c-Met degradation. (a) HCC827-GR cells were treated with Lico A for 48 h, followed by incubation with MG132 for another 6 h. Cell lysates were subjected to co-immunoprecipitation (co-IP) analysis. (b) HCC827-GR cells were transfected with si-c-Cbl for 24 h, followed by treated with Lico A for 48 h. Cells were incubated with MG132 for 6 h, WCE was collected and subjected to IP and IB analysis. (c and d) HCC827-GR cells were transfected with si-c-Cbl for 24 h, followed by treated with Lico A for 48 h. Cell viability (c) and colony formation (d) was analyzed by MTS and soft agar assay, respectively. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. (e–g) HCC827-GR cells were transfected with si-c-Cbl for 24 h, followed by treated with Lico A for 48 h. WCE was subjected to IB analysis (e), trypan blue exclusion assay (f) and Caspase-3 Assay Kit (g) was used for live cell population examination and caspase-3 activity measurement, respectively. ∗p < 0.05 and ∗∗∗p < 0.001.