Figure 6.

Multiple alignment (a) and phylogeny (b) of arsenate-reductase gene (arsC) sequences identified in the strain Citrobacter youngae IITK SM2. Multiple alignments of arsC sequences (arsC1, arsC2, and arsC3) of IITK SM2 were performed using CLUSTAL_W. Nucleotides conserved in all the three arsC genes are highlighted in yellow. The neighbor-joining method [57] was used for developing distance-based phylogeny of arsC genes. For this phylogeny, arsC genes in strain IITK SM2 were compared with other As(V)-reducing organisms (excluding strains of Citrobacter). Percentage bootstrap values corresponding to 1000 replicates are shown next to the branches. The tree shown in (b) was drawn to scale.