Figure 4.

Solamargine impeded PCa tumor growth in vivo. (a) PC3 cells (1 × 106) were subcutaneously injected into nude mice (n = 6). Mice were treated with dimethyl sulfoxide (DMSO) or solamargine (SM) when the xenograft size reached 50 mm³. Dose schedules were DMSO or solamargine (5 mg/kg, intraperitoneal, once per 2 days for 4 weeks). Mice were sacrificed and tumor volume is shown. (b) The weights of the xenografts are shown. Error bars represent mean ± standard deviation (Mann-Whitney test; n = 6; ^∗∗∗P < 0.001). (c) Representative images of hematoxylin-eosin (HE) staining and IHC staining in xenografts (scale bar: 100 μm). (d) p-Akt and Ki67 expression is presented as the number of positive cells (Mann–Whitney test).