a-c, HLE, SNU-886 and MDA-MB-231 cells carrying doxycycline (Dox) inducible shLSD1 #1 were treated with 1 μg/mL of Dox, and relative cell number was measured. Data are represented as mean ± SD, n = 5 for HLE, n = 6 for SNU-886 and MDA-MB-231, two-way ANOVA.
d-f, HLE, SNU-886 and MDA-MB-231 cells were treated with different concentrations of LSD1 inhibitor SP-2577 and vehicle (DMSO), and relative cell number was measured. Data are represented as mean ± SD, n = 6, two-way ANOVA.
g, Tumor size of MDA-MB-231 xenograft in NSG mice treated with vehicle or SP-2577. Data are represented as mean ± SD, n = 6, two-way ANOVA.
h, Images of tumors derived from the MDA-MB-231 xenografts at the end of the experimental treatment.
i, Body weight of mice during the experimental procedure. No significant difference was found between vehicle and SP-2577 groups (data are presented as mean ± SD, n = 6, P = 0.72, two-way ANOVA).