Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat polyclonal Anti-Arp3 | Santa Cruz Biotechnology | sc-11973 |
| Mouse monoclonal anti-actin clone C4 | Sigma-Aldrich /Millipore | MAB1501 |
| Bacterial and virus strains | ||
| BL21-CodonPlus(DE3)-RIL | Agilent | Cat. #230245 |
| Biological samples | ||
| Acetone powder of rabbit skeletal muscle | Pel-Freeze Biologicals | Cat. #41995-2 |
| Chemicals, peptides, and recombinant proteins | ||
| Alexa Fluor 647 C2 maleimide | ThermoFisher Scientific | Cat. # A20347 |
| Oregon Green 488 maleimide | ThermoFisher Scientific | Cat. # O6034 |
| N-(1-pyrene)iodoacetamide | ThermoFisher Scientific | Cat. #P29 |
| PEG succinimidyl succinate, MW5000 | JenKem | Cat. # A3011-1 |
| EZ-Link-NHS-PEG12-biotin | ThermoFisher Scientific | Cat. # 21312 |
| biotin-PEG NHS ester, MW5000 | JenKem | A5027-1 |
| NeutrAvidin | ThermoFisher Scientific | Cat.# 31000 |
| cOmplete, EDTA-free Protease Inhibitor Cocktail tablets | Sigma | Cat.# 11873580001 |
| Catalase | Sigma | Cat.# C3515 |
| Glucose Oxidase | MP Biomedicals | Cat.#195196 |
| Myosin II, rabbit skeletal muscle | Cytoskeleton | Cat # MYO2 |
| Albumin, from bovine serum | Sigma | Cat # A2153 |
| Critical commercial assays | ||
| Deposited data | ||
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| S. cerevisiae: BN020, Genotype: MAT?, ura3-52, his3-Δ200, leu2-3, lys2-801, trp1-1, Δarp2::TRP1, Δarp3::HIS3 pBN002(ARP3)::LEU2 pBN001(ARP2)::LYS2 | 28 | N/A |
| S. cerevisiae: BN021, Genotype: MAT?, ura3-52, his3-Δ200, leu2-3, lys2-801, trp1-901, Δarp2::TRP1 Δarp3::HIS3 pBN009(arp3 L155C)::LEU2 pBN003(arp2 R198C)::LYS2 | 28 | N/A |
| S. cerevisiae: BN283, Genotype: pep4::kan | Gift from Tom Stevens | N/A |
| Oligonucleotides | ||
| Recombinant DNA | ||
| pGEX-N-WASp-WA | 52 | Plasmid #22 |
| pGV67_SpDip1 | 3 | Plasmid #174 |
| pRSF Duet 1 (MmCPα1β2) | 58 | Plasmid #147 |
| pMW172-HsCofilin | gift from David Kovar | Plasmid #371 |
| pBH_Cdc42_Q61L | gift from Ken Prehoda | Plasmid #350 |
| pGV67_Crn-WD-CC | 32 | Plasmid #90 |
| pGV67-Hs-WASP-pVCA | gift from Rajaa Boujemaa-Paterski, recloned into pGV67 | Plasmid #335 |
| pGV67-GST-Las17-VCA | 32 | Plasmid #73 |
| pMW Human PRF1 | gift from David Kovar32 | Plasmid #359 |
| Software and algorithms | ||
| ImageJ | NIH, version 1.53c, open-source software | https://imagej.nih.gov/ij/; RRID:SCR_002285 |
| ImageJ filament tracking plugin | gift from Jeff Kuhn | N/A |
| Imaris v9.7.2 | Bitplane | http://www.bitplane.com/imaris/imaris;RRID:SCR_007370 |
| ImageStudioLite software | LI-COR Biosciences | https://www.licor.com/bio/image-studio-lite/; RRID:SCR_013715 |
| GraphPad Prism v9.2.0 | GraphPad Software | RRID:SCR_002798 |
| MATLAB | Mathworks | RRID:SCR_001622 |
| Other | ||