Antibodies |
goat anti-chicken Alexa Fluor 647 |
Life Technologies |
Cat#A-21449, RRID:AB_2535866 |
Tyrosine Hydroxylase Antibody cocktail |
Aves Labs |
Cat#TYH, RRID:AB_10013440 |
Anti-Dopamine Transporter (rabbit polyclonal) |
MilliporeSigma |
Cat#AB2231, RRID:AB_1586991 |
Monoclonal Beta-actin antibody |
MilliporeSigma |
Cat#A2228, RRID:AB_476697 |
Bacterial and Virus Strains |
AAV5-EF1α-DIO-EYFP |
UNC Vector Core |
Lot#AV4310K |
AAV5-CAG-FLEX-jGCaMP7b-WPRE |
Addgene |
Lot#18-429, RRID:Addgene_104497 |
AAV5-EF1α-DIO-hChR2(H134R)-EYFP |
Addgene |
Lot#v17652, RRID:Addgene_55639 |
AAV5-EF1α-DIO-eNpHR3.0-EYFP |
Addgene |
Lot#v32533, RRID:Addgene_26966 |
Chemicals, Peptides, and Recombinant Proteins |
Isoflurane |
Henry Schein |
N/A |
Buprenorphine SR |
Zoopharm |
Lot#1-212403 |
Carpofen |
Zoetis |
N/A |
Euthasol |
Virbac |
N/A |
Normal Goat Serum |
Jackson ImmunoResearch Laboratories |
Lot#153636, RRID:AB_2336990 |
Fluoromont-G |
Southern Biotech |
Cat#0100-01 |
Triton X |
Sigma |
Cat#X100-1L |
Critical Commercial Assays |
Pierce BCA protein assay kit |
Thermo Fisher |
Lot#WG332025 |
Deposited Data |
Photometry Analysis Code (MATLAB) |
Generated by study |
DOI: https://zenodo.org/badge/latestdoi/445620417
|
Experimental Models: Organisms/Strains |
Mouse: DAT-IRES-Cre: B6.SJL-Slc6a3tm1.1(cre)Bkmn/J |
Bred in house |
N/A |
Mouse: WT: C57BL/6J |
Jackson Laboratories |
Strain #000664, RRID:IMSR_JAX:000664 |
Software and Algorithms |
Synapse |
Tucker Davis Technologies |
https://www.tdt.com/component/synapse-software/
|
ImageJ |
51
|
https://imagej.nih.gov/ij/, RRID:SCR_003070 |
MED-PC V |
Med Associates |
https://www.med-associates.com/med-pc-v/, RRID:SCR_014721 |
MATLAB |
Mathworks |
https://www.mathworks.com/products/matlab.html, RRID:SCR_001622 |
Ethovision XT |
Noldus |
https://www.noldus.com/ethovision-xt, RRID:SCR_000441 |