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. 2022 Mar 10;29(3):190–193. doi: 10.1038/s41594-022-00729-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — Multi-body refinement of the UMOD filament core plus D10C domain (left path) and the whole UMOD branch (right path) were performed separately. Helical symmetry was applied to the filament core plus D10C, after the best homogenous filamentous segments were selected based on 2D classes. Meanwhile, the particles with the better contrast, more extended branch features were independently selected, locally 3D classified and refined, without helical symmetry. The final composite map was assembled by merging copies of the branch with the filament core plus D10C.