Extended Data Fig. 4. Mutants in seipin’s lumenal central helix retain function in vivo.
(a) Western blot analysis of seipin expression level. Cells expressing WT or indicated mutant constructs with C-terminal 13xmyc tag from the endogenous promoter. Sei1 detected with anti-myc antibodies. (b,c) Quantification of images shown in Fig. 2c. n=3 biologically independent experiments. (d) Growth of yeast strain sei1∆ carrying vectors with C-terminally GFP-tagged SEI1 sequences or empty vector on synthetic medium ± 100 µg/ml terbinafine. (e) Western blot analysis of whole-cell-lysates from strains in d using antibodies against GFP to detect seipin, against Ldb16 or Pgk1 as loading control. Representative immunoblots of two biologically independent experiment repeats is shown.