Reagent	Dose/Concentration (if applicable)	Company	Catalog Number
C57BL/6J mice		Jackson Laboratories	000664
Head Plate		Narishige	CP-1
VetBond		3M	006245
Dental Acrylic		Lang Dental Mfg. Co. Inc.	1334
Trephine drill bit		Fine Science Tools	18004-27
Borosilicate Glass Capillaries	Used to make glass pipettes	Kwik-Fil	18150F-4
Isofluorane	3–4% induction, 1-2% maintenance	Piramal	66794-017-10
Alpha Chloralose	5mg/ml in 20% PEG. Inject 50 ug/g mouse weight	Sigma	23120
PEG; Polyethylene glycol	20% solution in saline	Fluka	81300
Fluorescein Dextran, 70,000 MW, Anionic	5% stock solution in saline. Inject 65 μl in tail vein.	Invitrogen	D1823
Rhodamine Dextran, 10,000 MW, Anionic	5% stock solution in saline. Dilute to 0.5% with drug/vehicle for picospritz.	Invitrogen	D1824
AlexaFluor 633 hydrazide	1mg/ml stock solution in saline. Inject 25 μl in tail vein.	Invitrogen	A30634
L-Glutamic acid	100 μM and 1mM in ACSF. Equimolar equivalent of NaOH. pH = 7.4	Sigma	G1251
D-Aspartic acid	100 μM and 1 mM in ACSF. Equimolar equivalent of NaOH. pH = 7.4	Sigma	219096
L-THA; L-(-)-threo-3-Hydroxyaspartic acid	100 μM and 1 mM in ACSF Equimolar equivalent of NaOH. pH = 7.4	Tocris	0183
[1.2pt] TFB-TBOA; (3S)-3-[[3-[[4-(Trifluoromethyl) benzoyl]amino]phenyl] methoxy]-L-aspartic acid	1 μM in ACSF Equimolar equivalent of NaOH, pH = 7.4	Tocris	2532
KB-R7943; 2-[2-[4-(4-Nitrobenzyloxy)phenyl]ethyl] isothiourea mesylate	15 μM in 0.1% DMSO in ACSF	Tocris	1244
[1.2pt] YM-244769; N-[(3-Aminophenyl)methyl]-6-[4-[(3-fluorophenyl)methoxy] phenoxy]-3-pyridinecarboxamide dihydrochloride	1 μM in 0.1% DMSO in ACSF	Tocris	4544
DNQX; 6,7-Dinitroquinoxaline-2,3-dione	10 μM in ACSF	Tocris	2312
D-APV; D-(-)-2-Amino-5-phosphonopentanoic acid	50 μM in ACSF	Tocris	0106
MCPG; (RS)-α-Methyl-4-carboxyphenylglycine disodium salt	1 mM in ACSF	Tocris	3696
R-Baclofen; (R)-4-Amino-3-(4-chlorophenyl)butanoic acid	100 μM in ACSF	Tocris	0796
BAPTA; 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)	200 μM in ACSF	Tocris	2787
NPA; N5-[Imino(propylamino)methyl]-L-ornithine hydrochloride	2 μM in ACSF	Tocris	1200
Indomethacin; 1-(4-Chlorobenzoyl)-5-methoxy-2-methyl-1H-indole	10 μM in 0.1% DMSO in ACSF	Tocris	1708
Dimethyl sulfoxide (DMSO)		Sigma	34869