Figure 5.

Inhibition of TGF-β1 by SB431542 suppressed T cells activity by inhibiting PD-L1 glycosylation in Vitro. (A, B) The death rate of 5-8F or CNE1 cells co-cultured with Jurkat T cells for 6h. The tumor cells were STT3A-kncokdowned by shRNA or pretreated with SB431542 (20μM) or TM (10μg/ml) for 48h. (C, D) IL-2 production by activated Jurkat T cells co-cultured with 5-8F or CNE1 cells with shSTT3A or pretreated with SB431542 (20μM) or TM (15μg/ml) for 48h. IL-2 secretion was measured by ELISA. Bars, mean ± SD, **p<0.01.