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. 2022 Mar 4;12:847543. doi: 10.3389/fonc.2022.847543

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Copyright © 2022 Elemam, Youness, Hussein, Shihab, Yakout, Elwany, Manie, Talaat and Maghazachi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical assessment of GPR68 expression in paraffin-embedded breast cancer tissues. (A) Microscopic images showing various degrees of intensity: mild, moderate, and strong GPR68 expression. Images were captured at x400 magnification, with a scale bar representing 100 μm. Brown/DAB staining denotes GPR68 expression. (B) Immunoreactive scoring of GPR68 expression in immunohistochemical staining of BC tissues compared to non-tumor tissues. (C) GPR68 expression according to immunoreactive scores between the different molecular subtypes of breast cancer patients. (D) Semi-quantitative assessment of GPR68 expression in breast cancer patients compared to non-tumor breast tissues, by calculating the optical density of DAB substrate using IHC toolbox-Image J. (E) Semi-quantitative assessment of GPR68 expression across the different molecular subtypes of breast cancer patients. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.