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. 2022 Mar 18;13(3):249. doi: 10.1038/s41419-022-04685-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Left, the representative staining images of CRC specimens and adjacent tissues; right, quantification of the staining intensity using Image-Pro PLUS (v.6) and Image J (NIH) computer software. ***p < 0.001. a significant difference between groups as indicated. B Immunoblot (IB) analysis of Skp2 protein level in Skp2 knock out CRC stable cell lines. C MTS assay was performed to determine the cell viability of Skp2 knock out CRC stable cell lines treated with/without 2 Gy irradiation (IR). ***p < 0.001. D, E Plate colony formation assay analysis of the colony formation of Skp2 knock out HCT116 (D) and HT29 (E) stable cell lines treated with/without IR (2 Gy). ***p < 0.001. F, G Soft agar assay determination of the anchorage-independent cell growth of Skp2 knock out HCT116 (F) and HT29 (G) stable cell lines treated with/without IR (2 Gy). ***p < 0.001. H–J Average tumor volume (H), average tumor weight (I), and the population of Ki67 positive cells (J) of HCT116-sgCtrl and HCT116-sgSkp2 xenografts treated with/without IR. ***p < 0.001.