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. 2022 Mar 18;13(3):249. doi: 10.1038/s41419-022-04685-0

Fig. 3. Irradiation decreases Mcl-1 protein level.

Fig. 3

A Skp2 knockout CRC stable cells were treated with/without IR (2 Gy) and cultured for 72 h, WCE was subjected to IB analysis. B–D HCT116, and HT29 cells were transfected with siMcl-1 for 24 h, followed by IR (2 Gy) treated and cultured for 72 h. WCE was subjected to IB analysis (B). The live cell population was determined by trypan blue exclusion assay (C). Caspase 3 activity was examined by Caspase 3 Assay Kit (D). ***p < 0.001. E–H Mcl-1 was transiently transfected into Skp2 depleted HCT116 and HT29 cells using lipofectamine 2000 and maintained for 24 h. Cells were treated by IR (2 Gy) and cultured for 72 h. WCE was subjected to IB analysis (E). Cell viability and live cell population were determined by MTS assay (F) and trypan blue exclusion assay (G), respectively. Caspase 3 activity was examined by Caspase 3 Assay Kit (H). **p < 0.01, ***p < 0.001.