Skip to main content
. 2022 Mar 18;13(3):249. doi: 10.1038/s41419-022-04685-0

Fig. 6. Irradiation inhibits in vivo tumor growth.

Fig. 6

A–C Mcl-1 5KR reintroduction into Mcl-1 knockdown HCT116 cells rescues tumorigenesis under IR treatment. Mcl-1 5KR mutant was reintroduced into Mcl-1 knockdown HCT116 cells by lipofectamine 2000. The positive colonies were selected by G418 for 3 weeks, followed by injection into nude mice to establish the xenograft mouse model. Tumor size was monitored (A). Tumors were weighed (B). Ki67 positive cells were examined by IHC staining (C). Scale bar, 50 µm. **p < 0.01, ***p < 0.001. D–F Knockdown of FBW7 in Skp2-null HCT116 cells rescues tumorigenesis under IR treatment. FBW7 was silenced in Skp2-null HCT116 cells and injected into nude mice to establish the xenograft mouse model. Tumor size was monitored (D). Tumors were weighed (E). Ki67 positive cells were examined by IHC staining (F). Scale bar, 50 µm. **p < 0.01, ***p < 0.001.