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. 2022 Mar 17;5:235. doi: 10.1038/s42003-022-03180-8

Fig. 1. PDGF-BB:PDGFRβ signalling components are altered in the AD brain.

Fig. 1

Primary human brain pericytes and endothelial cells were isolated and PDGFRβ signalling components were measured by qPCR. a qPCR of lineage markers (PECAM1 and ANPEP) and PDGF-BB:PDGFRβ signalling components in isolated human brain endothelial cells and pericytes. N = 6–8. **p < 0.01, ***p < 0.001, unpaired t test. Tissue microarray blocks containing control and AD cores were stained with PDGFRβ (n = 41–42), or UEA lectin to label blood vessels and RNAscope probes against PDGFB and PDGFRB (n = 14–28). b Confocal images of PDGFB and PDGFRB probes relative to lectin in epilepsy biopsy cortex. Scale bar = 100 μm, inset = 10 μm. c Representative immunohistochemical staining for PDGFRβ in control and AD cortex, and quantification of staining intensity. Scale bar = 100 μm. d Representative images of PDGFB and PDGFRB ISH in AD and control cortex. Scale bar = 100 μm. e Expression of PDGFB and PDGFRB in bulk RNAseq data of control and AD temporal cortex, derived from Allen et al. 51. n = 80, unpaired t test. f PDGFRB expression in frozen temporal cortex tissue from control and AD brains, detected by Nanostring. n = 9, unpaired t test. g Quantification of PDGFB and PDGFRB puncta associated with lectin-positive blood vessels. n = 14–21, unpaired t test.