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. 2022 Mar 17;5:235. doi: 10.1038/s42003-022-03180-8

Fig. 8. PDGF-BB alters pericyte inflammatory secretions.

Fig. 8

Pericytes were treated with PDGF-BB (10 ng/mL) or vehicle for up to 96 h, RNA extracted at endpoint and qPCR performed. a Kinetics of CCL2, CXCL8, IL6 and CX3CL1 induced by PDGF-BB expression. n = 4, two-way ANOVA. Pericytes were treated with PDGF-BB (10 ng/mL) or vehicle for up to 96 h, then fixed at endpoint and conditioned media collected. Samples were then immunostained and secretions in conditioned media analysed by cytometric bead array. b Quantification of MCP-1, IL-8, IL-6 and CX3CL1 secretion and c MCP-1 immunostaining in pericytes treated with PDGF-BB. n = 3, two-way ANOVA. Scale bar = 100 μm, insert = 10 μm. *p < 0.05, **p < 0.01, ***p < 0.001 vs vehicle control, #p < 0.05, ##p < 0.01, ###p < 0.001 vs PDGF-BB-treated.