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. 2022 Mar 17;12:4652. doi: 10.1038/s41598-022-08725-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal laser scanning microscopy image the enamel after: (A) Demineralization (control group), (B) Fissure sealant penetration into demineralized enamel treated with 37% phosphoric acid, (C) Infiltrant resin that penetrated into demineralized enamel, (D) Fissure sealant that penetrated into demineralized enamel treated with 15% hydrochloric acid. Red: Penetration zone of methyl-rhodamine isocyanate (diffusion zone). Green: Penetration zone of sodium fluorescein (demineralized zone).