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. 2022 Mar 17;13:1413. doi: 10.1038/s41467-022-28744-4

Fig. 4. SP@AMF shows comprehensive protective effect overall small intestine against radiation-induced early injury.

Fig. 4

a Schematic illustration of the experiment protocol. b, c Represented IHC images (b) and the quantification (c) of the regenerating crypts in the small intestine (duodenum, jejunum, and ileum) stained by Ki67 at day 3 after being treated by sham irradiation + PBS (PBS group), 12 Gy abdominal X-ray (IR) + PBS, IR + SP, IR + AMF, and IR + SP@AMF (n = 6 biologically independent animals). The black dotted line indicates the Ki67-stained regenerating crypts. Scale bar =100 µm. Experiment was repeated three times independently with similar results. d Quantification of the length of intestinal villi (n = 6 biologically independent animals). The represented HE images are shown in Supplementary Fig. 17. e Quantification of the γH2AX-positive intestinal cells (n = 6 biologically independent animals). The represented IHC images are shown in Supplementary Fig. 18. f–h Pro-inflammatory cytokines including IL-1β (f), IL-6 (g), and TNF-α (h) in the small intestine tissue (n = 6 biologically independent animals). The data show means + SD. P was calculated using two-tailed t-test. *P versus IR + PBS group (*<0.05, **<0.01, ***<0.001, n.s., no significance).