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. 2022 Mar 17;6:16. doi: 10.1038/s41698-022-00260-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of the workflow to establish lorlatinib intermediate resistant cells from JFCR-028-3 parental cells, established after three cycles of drug treatment and a drug holiday. Scale bar = 100 μm. b Cell viability of JFCR-028-3 parental and lorlatinib intermediate resistant cells (n = 3). Each point represents the mean ± SD of three replicates. c Western blot analysis showing suppression of phospho-ALK and its downstream signaling in JFCR-028-3 parental and lorlatinib intermediate resistant cells treated with indicated concentrations of lorlatinib for 3 h. GAPDH was used as a loading control.