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. 2022 Mar 17;13:1441. doi: 10.1038/s41467-022-29122-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a tSNE plots showing the distributions of selected immune clusters that are significantly different in abundance from PBMC (P), Non-tumour (N) or tumour (T) compartments across three TNM stages. b Graphs showing percentages of clusters with significant differences across TNM stages in P (blue boxes). c Graphs showing percentages of clusters with significant differences across TNM stages in N (green boxes). d Graphs showing percentages of clusters with significant differences across TNM stages in T (red boxes). e Graphs showing the percentages of manually gated immune populations from total live immune cells (by flowjo) in P, N and T across S1, S2 and S3 HCC. The bar and arrows indicate the major immune cell lineages shown below. a–d TNM stage I, II and III (S1, S2 and S3). n_PS1 = 13, n_PS2 = 12, n_PS3 = 9, n_NS1 = 12, n_NS2 = 12, n_NS3 = 9, n_TS1 = 13, n_TS2 = 12, n_TS3 = 9. b–e Phenotypes are indicated on top. b–e Boxplots show median and the whiskers represent minimum and maximum values with the box edges showing the first and third quartiles. One-way ANOVA test with two-sided p-values by unpaired two-sided Mann–Whitney U (MWU) tests for two-group comparisons. e n_PS1-S3 = 38, n_NS1-S3 = 38, n_TS1 = 16, n_TS2 = 12, n_TS3 = 10. Source data are provided as a Source Data file.