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. 2022 Mar 17;13:1423. doi: 10.1038/s41467-022-29068-z

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Quantification of BrdU⁺ and PDGFRα⁺ cells in the corpus callosum of Olig1-Cre; Setdb1^F/+ and Olig1-Cre; Setdb1^F/F mice at P14. b In situ hybridization of Enpp6⁺ in the corpus callosum at P14. n = 3 control and three mutant mice. c Quantification of Enpp6⁺ cells in the corpus callosum at P14. Error bars indicate SEM (p < 0.001. two-tailed unpaired Student’s t test). d Expression of Enpp6 in spinal cords of Setdb1 control and mutant mice (left panel) and rat iOLs transfected with Olig2 or Olig2-VP64 (right panel). Error bars indicate SEM (p < 0.001. two-tailed unpaired Student’s t test). e Immunostaining of MBP in the corpus callosum from NG2-Cre; Setdb1^F/+ and NG2-Cre; Setdb1^F/F mice at P14. n = 3 control and three mutant mice. f Quantification of CC1⁺ cell in the corpus callosum and spinal cord from NG2-Cre; Setdb1^F/+ and NG2-Cre; Setdb1^F/F mice at P14. Error bars indicate SEM (p < 0.001. two-tailed unpaired Student’s t test). g Schematic diagram showing markers of each stage in oligodendrocyte lineage. h Survival curve of Cnp-Cre; Setdb1^F/+ and Cnp-Cre; Setdb1^F/F mice. i Quantification of myelin sheath thickness (g-ratio) in optic nerves from Cnp-Cre; Setdb1^F/+ and Cnp-Cre; Setdb1^F/F mice at P14. n = 3 control and three mutant mice. j Quantification of CC1⁺ cells in the spinal cord of Cnp-Cre; Setdb1^F/+ and Cnp-Cre; Setdb1^F/F mice at P14. n = 3 control and three mutant mice. k Schematic diagram showing tamoxifen administration to Sox10-Cre^ER; Setdb1 Flox mice in two consecutive days at P3, followed by tissue collection at P15. n = 3 control and three mutant mice. l–o Immunostaining of MBP and CC1 in the corpus callosum (l, m) and spinal cord (n, o) from Sox10-Cre^ER; Setdb1^F/+ and Sox10-Cre^ER; Setdb1^F/F mice at P14. n = 3 control and three mutant mice. p Quantification of CC1⁺ cells in the white matter or gray matter of spinal cord. Error bars indicate SEM (p < 0.001. two-tailed unpaired Student’s t test). Scale bars: 100 μm in (b); 250 μm in (e and l–o).