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. 2022 Mar 4;13:848728. doi: 10.3389/fendo.2022.848728

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Copyright © 2022 Wang, Zhai, Lei, Xu, Jiang, Kuang, Zhang, Liu, Bian, Yang, Zan, Jin, Li and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Regulation of MCHR1 trafficking by WT MRAP2 and its mutants. (A) Surface expression of MCHR1 measured by ELISA assay, which transfected with MRAP2 at different ratios (from 1:0 to 1:9). (B) D-value of MCHR1 membrane surface expression in the control group (RAMP3) and MRAP2 group at different transfection ratios. (C) Total expression level of MCHR1. (D) Localization of GFP-MCHR1 by confocal microscopy, which transfected with empty vector (left) or MRAP2 (middle) or RAMP3 (right). (E) Sequence alignment of human and mouse MRAP2. (F) Schematic representation of the MRAP2 mutants constructed. (G) The surface expression of MCHR1 when co-transfected with empty vector (control), WT MRAP2, or different mutants. One-way ANOVA with post-hoc Tukey test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.