Table 1.
Characterization of reactions between mAbs and DENV NS1 proteins.
| Hybridoma | Isotype | Type of epitope | Reactivity of four DENV serotypes (DENV1-4) | Specificity | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Western blot a | ELISA b | |||||||||||
| D1 | D2 | D3 | D4 | D1 | D2 | D3 | D4 | |||||
| 82-1.1 | IgG1,κ | linear | + | + | + | + | + | + | + | + | NS1 | |
| 51-1.1 | IgG2b,κ | conformational | + | – | – | – | + | – | – | – | NS1 | |
| 33-7.1 | IgG1,κ | conformational | – | + | – | – | – | + | – | – | NS1 | |
| 43-1.3 | IgG2a,κ | conformational | – | – | + | – | – | – | + | – | NS1 | |
| 22-1.5 | IgG1,κ | conformational | – | – | – | + | – | – | – | + | NS1 | |
a
The lysates of C6/36 cells infected with different dengue virus serotypes were treated using SDS-PAGE sample buffer and then subjected to gel electrophoresis before being transferred to a nitrocellulose membrane and blotted with each mAb.
b
Different NS1 antigens were immunoaffinity-purified from cell culture supernatants of Vero cells infected with different serotypes of DENV. Microwell plates were coated with specific NS1 antigens and reacted with each mAb.