Table 6.

Performance of DENV serotype NS1 multiplex LFIA in detection of NS1 in acute-phase sera.

Virus	Reference test	Total number of serum samples (n=187)		Number of serum samples that tested positive using DENV serotype NS1 multiplex LFIA
				D1 test line	D2 test line	D3 test line	D4 test line	Overall
DENV1 a	Serotype RT-PCR	30	total DENVs: 105	27	2	0	0	total: 91
DENV2 a	Serotype RT-PCR	33		1 g	29	1 g	1 g
DENV3 a	Serotype RT-PCR	23		0	0	19	1
DENV4 a	Serotype RT-PCR	18		0	0	0	15
DENVs b	NS1 AG ELISA/Dengue IgM/IgG capture ELISA	1		0	1	0	0
ZIKV c	RT-PCR/sequence	3		0	0	0	0	0
JEV d	RT-PCR	5		1 h	1 h	0	0	1 h
CHIKV e	RT-PCR	5		0	0	0	0	0
Unknown f	RT-PCR/ELISA/Dengue IgM/IgG capture ELISA/NS1 AG	69		0	3 i	0	3 i	4 i

^aA positive result was obtained using Dengue serotype-specific 1-step SYBR Green I-based real-time RT-PCR.

^bA negative result was obtained using Dengue serotype-specific 1-step SYBR Green I-based real-time RT-PCR, whereas a positive result was obtained using Platelia Dengue NS1 AG ELISA, Dengue NS1 ELISA for serotype, and dengue virus specific IgM/IgG capture ELISA.

^cA positive result was obtained using Flavivirus 1-step SYBR Green I-based real-time RT-PCR and sequences identified as Zika virus.

^dA positive result was obtained using JEV specific 1-step SYBR Green I-based real-time RT-PCR.

^eA positive result was obtained using CHIKV specific 1-step SYBR Green I-based real-time RT-PCR.

^fA negative result was obtained using flavivirus and alphavirus 1-step SYBR Green I-based real-time RT-PCR, flavivirus and alphavirus specific IgM/IgG capture ELISA, Platelia Dengue NS1 AG ELISA, and/or Dengue NS1 ELISA for serotype.

^gD1, D3, and D4 test lines presenting cross-reactivity with DENV2.

^hD1 and D2 test lines presenting weak cross-reactivity with JEV.

ⁱFour serum samples from unknown fever, two of which presented cross-reactivity with D2 and D4 test lines at the same time.