Table 9.
Validation results for DENV serotype NS1 multiplex LFIA for mosquitoes infected with flaviviruses or Chikungunya virus.
| Intrathoracic injection of virus in Aedes aegypti | N= | Test results using | |||||
|---|---|---|---|---|---|---|---|
| Real-time-PCR | DENV serotype NS1 multiplex LIFA | ||||||
| DENV mono-infection | D1 | D2 | D3 | D4 | |||
| DENV1 | 5 | 5/5 D1 positive | 5/5 | + | – | – | – |
| DENV2 | 5 | 5/5 D2 positive | 5/5 | – | + | – | – |
| DENV3 | 5 | 5/5 D3 positive | 5/5 | – | – | + | – |
| DENV4 | 5 | 5/5 D4 positive | 5/5 | – | – | – | + |
| DENV co-infection | |||||||
| DENV1/DENV2 | 5 | 5/5 D1/D2 positive | 5/5 | + | + | – | – |
| DENV1/DENV3 | 5 | 5/5 D1/D3 positive | 5/5 | + | – | + | – |
| DENV1/DENV4 | 5 | 5/5 D1/D4 positive | 5/5 | + | – | – | + |
| DENV2/DENV3 | 5 | 5/5 D2/D3 positive | 5/5 | – | + | + | – |
| DENV2/DENV4 | 5 | 5/5 D2/D4 positive | 5/5 | – | + | – | + |
| DENV3/DENV4 | 5 | 5/5 D3/D4 positive | 5/5 | – | – | + | + |
| Other flaviviruses | |||||||
| Zika virus | 5 | 5/5 ZIKV positive | 5/5 | – | – | – | – |
| West Nile virus | 5 | 5/5 West Nile positive | 5/5 | – | – | – | – |
| Japanese encephalitis virus | 5 | 5/5 JEV positive | 5/5 | – | – | – | – |
| Yellow fever virus | 5 | 5/5 YF positive | 5/5 | – | – | – | – |
| Alphavirus | |||||||
| Chikungunya virus | 5 | 5/5 CHIKV positive | 5/5 | – | – | – | – |