Fig. 4.

Compositional changes to the postmating sperm proteome. (A and B) Venn diagrams indicating the overlap and number of canonical sperm proteins, SFPs, and female-derived proteins in the transferred sperm proteome (bursa 30 min after mating) (A) and stored sperm proteome (seminal receptacle [SR] 2 h after mating) (B). (C) Relative abundance of the nonoverlapping protein categories at each collection point. Relative abundances were calculated from the summed NSAFs for each protein category at the respective collection point. (D) Number of reproductive gland proteins identified at 30 min, 2 h, and 4 d after mating. (E) Abundance comparisons of reproductive gland proteins at 30 min and 2 h after mating. (F) Correlation between female contribution and the change in abundance after storage among dual-derived sperm proteins (Pearson’s r = 0.71; P = 0.001).