Table 1.
Primer sequences used in qRT-PCR.
| Primers | Forward primer 5’-3’ | Reverse primer 3’-5’ | GenBank accession n° |
|---|---|---|---|
| MMP-9 | TGTACGGACCCGAAGC | CCGTCCTTATCGTAGTCAG | NM013599 |
| MMP-12 | TTTGACCCACTTCGCC | GTGACACGACGGAACAG | AK089523 |
| TIMP-1 | CCA CGA ATC AAC GAG ACC | GGC CCG TGA TGA GAA AC | NM011593 |
| NF-kB | TCCGGGAGCCTCTAGTGAG | TCCATTTGTGACCAACTGAACGA | NM_008689.2 |
| TLR-2 | GAGCATCCGAATTGCATCACC | CCCAGAAGCATCACATGACAGAG | NM_011905.3 |
| TLR-4 | CATGGATCAGAAACTCAGCAAAGTC | CATGCCATGCCTTGTCTTCA | NM_021297.2 |
| GAPDH | CACTCACGGCAAATTCAACGGCAC | GACTCCACGACATACTCAGCAC | AA661116.1 |
| β-actin | TGCTGTCCCTGTAGTCCTCT | AGGTCTTTACGGATGTCAACG | NM 007393.1 |
MMP-9, Matrix Metalloproteinases-9; MMP-12, Matrix Metalloproteinases-12; TIMP-1, Tissue Inhibitor Matrix Metalloproteinase-1; NF-kB, Nuclear Factor kappa B; TLR-2, Toll-Like Receptor-2; TLR-4, Toll-Like Receptor-4; GAPDH, Glyceraldehyde-3 Phosphate Dehydrogenase; β-actin, Beta-Actin Gene.