Figure 2.

Bivalent BL1.2 V_HH construct induces agglutination of F4⁺ ETEC in vitro and prevents ETEC adhesion to small intestinal pig enterocytes ex vivo

(A) Schematic representation and microscopy images of F4⁺ ETEC incubated with PBS, monovalent BL1.1, and bivalent BL1.2 V_HH construct.

(B) Microscopy images of freshly isolated small intestinal pig enterocytes incubated with PBS, BL1.1, and BL1.2.

(C) Percentage of pig enterocytes with two or more, one, or without bound F4⁺ ETEC bacteria, respectively. Enterocytes were added to bacteria exposed to the following treatments: no V_HH (PBS), BL1.1, BL1.2, or culture media alone. The number of attached F4⁺ ETEC bacteria to each pig enterocyte was quantified from counting of 50–150 enterocytes per treatment group.