Figure 3.

Evaluation of the amount of BL1.1 V_HH construct required to occupy all binding epitopes on F4⁺ETEC

(A) Schematic presentation of the ELISA setup used for determining the number of BL1.1 binding sites on a single F4⁺ ETEC bacterium.

(B) ELISA-based binding analysis of the monovalent BL1.1 V_HH construct to F4⁺ ETEC cells grown in log or stationary phase. The assay was used to estimate the BL1.1 concentration required to occupy all fimbrial epitopes present on a single F4⁺ ETEC cell. Binding assays (B) were performed as technical quadruplicates (n = 4), and data are presented as mean + SD.