REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Untagged BL1.1 and BL1.2 constructs	Novozymes laboratories, Bagsværd, Denmark	N/A
Mouse monoclonal anti-FLAG M2-Peroxidase (HRP) antibody	Sigma	A8592-5X1MG; RRID: AB_439702
Bacterial and virus strains
E. coli BL21 for cloning and expression	New England Biolabs	C2527H
Biological samples	This paper	N/A
Porcine gastric juice	This paper	N/A
Porcine bile	This paper	N/A
Chemicals, peptides, and recombinant proteins
NcoI restriction enzyme	New England Biolabs	R3193S
NotI restriction enzyme	New England Biolabs	R3189S
T4 DNA ligase	New England Biolabs	M0202S
Benzonase Nuclease	Millipore	E1014-25KU
cOmplete Protease Inhibitor Cocktail	Roche	11836145001
r-lysozyme	Sigma Aldrich	L6876-10G
SimplyBlue™ SafeStain	Invitrogen	10432072
LysC (MS grade)	Wako	121-05063
Trypsin (MS grade)	Promega	V5280
Critical commercial assays
PureLink™ Genomic DNA Mini Kit	Invitrogen	K182001
Kapa HyperPlus Library Prep Kit	Roche Molecular Systems	KK8514
MiSeq V2 300 Cycles Reagent kit	Illumina	MS-102-2002
Deposited data
Genome sequence data for the AU-F4 strain	This paper	N/A
Genome sequence data for the AU-F18 strain	This paper	N/A
Experimental models: Cell lines
Intestinal villous enterocytes	This paper	N/A
Experimental models: Organisms/strains
E. coli O149:F4 (9910045-1) Denoted as: F4+ ETEC/AUF4	Danish Veterinary Institute (Copenhagen, Denmark) (Frydendahl, 2002)	GenBank: JAKLOV000000000
E. coli O138:F18 (9910297-2STM) Denoted as: F18+ ETEC/AUF18	Danish Veterinary Institute (Copenhagen, Denmark) (Frydendahl, 2002)	GenBank: JAKLOW000000000
F4+ ETEC challenged weaning piglets	University of Aarhus, Foulum, Denmark	N/A
Oligonucleotides
BL1.1 (DNA)	Eurofins Genomics (Ebersberg, German) and Integrated DNA Technologies (Belgium)	N/A
BL1.2 (DNA)	Eurofins Genomics (Ebersberg, German) and Integrated DNA Technologies (Belgium)	N/A
Ctrl VHH (DNA)	Eurofins Genomics (Ebersberg, German) and Integrated DNA Technologies (Belgium)	N/A
Recombinant DNA
pSANG10-3F	Ahmadi et al. (2020)	N/A
Software and algorithms
SPAdes v. 3.11.1	http://cab.spbu.ru/software/spades/	N/A
QUAST	http://quast.sourceforge.net/docs/manual.html	N/A
SerotypeFinder 1.1	https://cge.cbs.dtu.dk/services/SerotypeFinder/	N/A
MLST 1.8	https://cge.cbs.dtu.dk/services/MLST/	N/A
BLASTn	https://blast.ncbi.nlm.nih.gov/Blast.cgi	N/A
Q-Exactive instrument	ThermoFisher Scientific	N/A
OriginPro version 2021b	OriginLab	N/A
Other
HisTrap FF 1 mL column	GE Life Science	GE17-5319-01
SDS-PAGE (NuPAGE 4-12% gels)	Invitrogen	NP0322BOX
C18 trap column	ThermoScientific	164705
C18 reversed-phase analytical column	ThermoScientific	ES803
Maxisorp plates (Nunc)	ThermoFisher	44-2404-21