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. 2022 Mar 11;119(11):e2118300119. doi: 10.1073/pnas.2118300119

Fig. 4.

Fig. 4.

The EBV latency III program confers resistance to ferroptosis-inducing agents. (A) BODIPY MFI + SD values from n = 3 independent replicates of MUTU I versus III BL cells treated with erastin (10 μM) and/or Fer-1 (5 μM) for 18 h and measured by FACS. (B) %7-AAD+ mean + SD values from n = 3 replicates of MUTU I versus III BL cells treated with erastin (10 μM) and/or Fer-1 (5 μM) for 24 h and measured by FACS. (C) BODIPY MFI + SD values measured by FACS of n = 3 independent replicates of MUTU I versus III BL cells treated with ML-210 (1 μM) and/or Fer-1 (5 μM) for 18 h as indicated. (D) %7-AAD+ mean + SD values measured by FACS of n = 3 replicates of MUTU I versus III BL cells treated with ML-210 (1 μM) and/or Fer-1 (5 μM) for 24 h and measured by FACS. (E) Dose–response curves of cells with EBV latency I to erastin for 24 h. Mean + SD of CellTiter-Glo live cell numbers from n = 3 replicates. IC50 values are shown at Right. (F) Dose–response curves of cells with EBV latency III to erastin for 24 h. Mean + SD of CellTiter-Glo live cell numbers from n = 3 replicates. IC50 values are shown at Right. (G) Dose–response curves of cells with EBV latency I to ML-210 for 24 h. Mean + SD of CellTiter-Glo live cell numbers from n = 3 replicates. IC50 values are shown at Right. (H) Dose–response curves of cells with EBV latency III to ML-210 for 24 h. Mean + SD of CellTiter-Glo live cell numbers from n = 3 replicates. IC50 values are shown at Right. (I) Dose–response curves of P-493 cells grown in the MYC-high Burkitt-like state (blue) or EBNA2-driven latency III state (red) to erastin for 24 h. Mean + SD of CellTiter-Glo live cell numbers from n = 3 replicates. IC50 values are shown at Right. (J) Dose–response curves of P-493 cells grown in the MYC-high Burkitt-like state (blue) or EBNA2-driven latency III state (red) to ML-210 for 24 h. Mean + SD of CellTiter-Glo live cell numbers from n = 3 replicates. IC50 values are shown at Right. P values were determined by one-sided Fisher’s exact test. *P < 0.05, **P < 0.005, ***P < 0.0005.