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. 2022 Feb 19;298(4):101749. doi: 10.1016/j.jbc.2022.101749

Figure 3.

Figure 3

STR18 stimulates ABA3 activity.A, effect of STR18 on ABA3 cysteine desulfurase (CD) activity. CD activity was measured using 1 μM ABA3, 500 μM l-cysteine, and 1 mM DTT in the presence or the absence of 5 μM STR18. The data are represented as mean ± SD of five independent experiments. B, steady-state kinetic parameters of the CD activity of ABA3 alone or in the presence of STR18 or its cysteinic variants. CD activity was measured in the presence of 1 μM ABA3, increasing concentrations of l-cysteine (0–1 mM), 5 mM DTT, and 5 μM STR18 when present. C, steady-state kinetic parameters of the CD activity of ABA3 in the presence of STR18. Activity was measured in the presence of 0.5 μM ABA3, 500 μM l-cysteine, 5 mM DTT, and increasing concentrations of STR18 (0–5 μM). The data are represented as mean ± SD of three independent experiments. STR18, sulfurtransferase 18.