Table 5.
Catalytic properties of characterized CD isoforms from various organisms
Protein name | Organism | Activity (nmol per min per mg) | KMl-cysteine (μM) | KM acceptor (μM) | Reference |
---|---|---|---|---|---|
NifS | Azotobacter vinelandii | 168 | — | — | (4) |
IscS | Azotobacter vinelandii | 124 | — | — | (4) |
SufS | Bacillus subtilis | 7 | — | — | (13) |
SufS–SufU | Bacillus subtilis | 240a | 86 | 3 | (13) |
SufS | Bacillus subtilis | (36) | |||
SufS–SufU | Bacillus subtilis | 153.5 | 49.7 | — | (36) |
SufS–SufU | Bacillus subtilis | 93.4 | — | 2.6 | (36) |
SufS | Erwinia chrysanthemi | 21 | — | — | (11) |
SufS–SufE | Erwinia chrysanthemi | 750 | — | — | (11) |
CsdA | Escherichia coli | 1.2 | 140 | — | (12) |
CsdA–CsdE | Escherichia coli | 2.5 | 540 | — | (12) |
IscS | Escherichia coli | 51.7b | — | — | (37) |
IscS | Escherichia coli | 312.8c | — | — | (37) |
IscS | Escherichia coli | 380 | — | — | (52) |
SufS | Escherichia coli | 19 | — | — | (52) |
SufS | Escherichia coli | 19 | — | — | (11) |
SufS–SufE | Escherichia coli | 900 | — | — | (11) |
SufS | Escherichia coli | 25 | — | — | (39) |
SufS–SufE | Escherichia coli | 700 | — | — | (39) |
SufS | Escherichia coli | 2.6b | — | — | (37) |
7.9c | — | — | (37) | ||
SufS–SufE | Escherichia coli | 54.3 | 43.5 | — | (37) |
SufS–SufE | Escherichia coli | 85.4 | — | 1.9 | (37) |
CD–Rhd | Pseudorhodoferax | 7600 | 310 | — | This study |
NFS1 | Homo sapiens | 6.4 | — | — | (53) |
NFS1–ISD11 | Homo sapiens | 435 | (42) | ||
NFS1 | Saccharomyces cerevisiae | 12.7 | — | — | (54) |
NFS2 | Arabidopsis thaliana | 13 | 100 | — | (16) |
NFS2–SUFE1 | Arabidopsis thaliana | 550 | 43 | — | (16) |
ABA3 | Arabidopsis thaliana | 16 | — | — | (19) |
SufS–SufU activity with 10-fold excess of SufU.
CD activity with 2 mM l-cysteine and 2 mM DTT.
CD activity with 12 mM l-cysteine and 50 mM DTT.