Table 5.
Catalytic properties of characterized CD isoforms from various organisms
| Protein name | Organism | Activity (nmol per min per mg) | KMl-cysteine (μM) | KM acceptor (μM) | Reference |
|---|---|---|---|---|---|
| NifS | Azotobacter vinelandii | 168 | — | — | (4) |
| IscS | Azotobacter vinelandii | 124 | — | — | (4) |
| SufS | Bacillus subtilis | 7 | — | — | (13) |
| SufS–SufU | Bacillus subtilis | 240a | 86 | 3 | (13) |
| SufS | Bacillus subtilis | (36) | |||
| SufS–SufU | Bacillus subtilis | 153.5 | 49.7 | — | (36) |
| SufS–SufU | Bacillus subtilis | 93.4 | — | 2.6 | (36) |
| SufS | Erwinia chrysanthemi | 21 | — | — | (11) |
| SufS–SufE | Erwinia chrysanthemi | 750 | — | — | (11) |
| CsdA | Escherichia coli | 1.2 | 140 | — | (12) |
| CsdA–CsdE | Escherichia coli | 2.5 | 540 | — | (12) |
| IscS | Escherichia coli | 51.7b | — | — | (37) |
| IscS | Escherichia coli | 312.8c | — | — | (37) |
| IscS | Escherichia coli | 380 | — | — | (52) |
| SufS | Escherichia coli | 19 | — | — | (52) |
| SufS | Escherichia coli | 19 | — | — | (11) |
| SufS–SufE | Escherichia coli | 900 | — | — | (11) |
| SufS | Escherichia coli | 25 | — | — | (39) |
| SufS–SufE | Escherichia coli | 700 | — | — | (39) |
| SufS | Escherichia coli | 2.6b | — | — | (37) |
| 7.9c | — | — | (37) | ||
| SufS–SufE | Escherichia coli | 54.3 | 43.5 | — | (37) |
| SufS–SufE | Escherichia coli | 85.4 | — | 1.9 | (37) |
| CD–Rhd | Pseudorhodoferax | 7600 | 310 | — | This study |
| NFS1 | Homo sapiens | 6.4 | — | — | (53) |
| NFS1–ISD11 | Homo sapiens | 435 | (42) | ||
| NFS1 | Saccharomyces cerevisiae | 12.7 | — | — | (54) |
| NFS2 | Arabidopsis thaliana | 13 | 100 | — | (16) |
| NFS2–SUFE1 | Arabidopsis thaliana | 550 | 43 | — | (16) |
| ABA3 | Arabidopsis thaliana | 16 | — | — | (19) |
a
SufS–SufU activity with 10-fold excess of SufU.
b
CD activity with 2 mM l-cysteine and 2 mM DTT.
c
CD activity with 12 mM l-cysteine and 50 mM DTT.