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. 2022 Feb 19;298(4):101748. doi: 10.1016/j.jbc.2022.101748

Figure 3.

Figure 3

Cell-autonomous TGF-β1 activity controls the lipogenic pathway in adipocytes.A and B, relative expression of the indicated genes after 12 h of TGF-β1 treatment in 3T3-L1 adipocytes (n = 4). C and D, relative expression of the indicated genes in 3T3-L1–tet–Tgfb1 adipocytes treated with 0.5 μg/ml doxycycline for 12 h (n = 4). E and F, relative expression of the indicated genes in 3T3-L1 adipocytes transfected with TGF-β1 siRNA (n = 3). G and H, relative expression of the indicated genes in 3T3-L1 adipocytes after 24 h of TGFBR inhibitor (SB431542: 10 μM) treatment (n = 4). Data are presented as the mean ± SEM. #p < 0.1, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. TGF-β1, transforming growth factor β1; TGFBR, transforming growth factor β receptor.