| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Critical commercial assays | ||
| QIAGEN Plasmid Maxi Kit | QIAGEN | Cat# 12162 |
| QIAGEN Plasmid Midi Kit | QIAGEN | Cat# 12143 |
| CalPhos Mammalian Transfection Kit | Takara Bio | Cat# 631312 |
| Chemicals, peptides, and recombinant proteins | ||
| Heat Inactivated-Fetal Bovine Serum (FBS) | Novus Biologicals | Cat# S11150H |
| Hanks’ Balanced Salt Solution (HBSS) | Thermo Fisher Scientific | Cat# 88284 |
| Minimum Essential Media (MEM) | Thermo Fisher Scientific | Cat# 11095080 |
| B-27 Supplement | Thermo Fisher Scientific | Cat# 17504044 |
| L-glutamine 200 mM (100×) | GenDepot | Cat# CA009-010 |
| Neurobasal Medium | Thermo Fisher Scientific | Cat# 21103049 |
| Poly-D-Lysine Solution | Thermo Fisher Scientific | Cat# A003E |
| Laminin I | R&D systems | Cat# 3400-010-02 |
| TrypLE Express Enzyme 1× | Thermo Fisher Scientific | Cat# 12604013 |
| 5-Fluoro-2′-deoxyuridine | MilliporeSigma | Cat# F0503 |
| Uridine | MilliporeSigma | Cat# U3003 |
| Penicillin/Streptomycin | Thermo Fisher Scientific | Cat# 15070063 |
| Triton X-100 | MilliporeSigma | Cat# T8787 |
| VECTASHIELD® Antifade Mounting Medium with DAPI | Vector Lab | Cat# H-1200-10 |
| Sodium pyruvate (100 mM) | Thermo Fisher Scientific | Cat# 11360070 |
| Paraformaldehyde | MilliporeSigma | Cat# 158127-500G |
| 2,2,2 Tribromoethanol | Fisher Scientific | Cat# AAA1870622 |
| 2-Methyl-2-butanol | Fisher Scientific | Cat# AAA18304AP |
| HCl | MilliporeSigma | Cat# 320331-500ML |
| NaOH | MilliporeSigma | Cat# 221465 |
| Normal Goat serum | Abcam | Cat# ab7481 |
| Acetic acid glacial | MilliporeSigma | Cat# 695092-100ML |
| Sucrose | MilliporeSigma | Cat# S0389-500G |
| Tissue-Plus™ O.C.T. Compound Tissue-Plus™ O.C.T. Compound | Fisher Scientific | Cat# 23-730-571 |
| Permount Mounting Medium | Electron Microscopy Sciences | Cat# 17986-01 |
| Nuclease-Free Water (not DEPC-Treated) | Fisher Scientific | Cat# 4387936 |
| Xylene | Fisher Scientific | Cat# X3S-4 |
| Software and algorithms | ||
| Fiji is Just ImageJ (Fiji) version 2.0.0-rc/1.5f | http://imagej.net/ | (Schindelin et al., 2015) |
| Neurolucida 360 | MBF Bioscience, Williston, VT | (Ayata et al., 2018) |
| Imaris | Oxford instruments | Cat# RRID:SCR_007370 |
| Zen2.6 Software | https://www.zeiss.com/microscopy/us/products/microscope-software/zen-core.html | Zeiss |
| Antibodies | ||
| Mouse monoclonal anti-HA | BioLegend | Cat# 901514 |
| Chicken polyclonal anti-GFP | Abcam | Cat# ab13970 |
| Donkey anti-mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 594 | Thermo Fisher Scientific | Cat# A32742 |
| Goat anti-Chicken IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 | Thermo Fisher Scientific | Cat# A32391 |
| Recombinant DNA | ||
| PUM1cDNA expression vector | OmickLink Expression Vector, GeneCopoeia | Cat# pEZ-M02, EX-E2337-M02-10 |
| pEGFP-C1 | Prof. Golenbock's Lab (UMass Chan Medical School, Worcester, Massachusetts 01605, USA) | (Gennarino et al., 2018) |
| Empty expression vector | OmickLink Expression Vector, GeneCopoeia | Cat# EX-NEG-M02 |
| OmickLinkTMExpression Vector pEZ-M02 | GeneCopoeia | Cat# EX-E2337-M02-10 |
| OmickLinkTMExpression Vector | GeneCopoeia | Cat# EX-NEG-M02 |
| Experimental models: Organisms/strains | ||
| B6/129 mixed background Pum1 mouse models. We used male and female mice. Neonatal mice for hippocampal neurons and at 10 weeks of age for Nissl staining | Prof. Haifan Lin’s Lab (Yale School of Medicine, New Haven, Connecticut 06520, USA) | (Gennarino et al., 2018) |
| Other | ||
| SouthernBiotech™ Gelatin Subbed Slides | Fisher Scientific | Cat# OBSLD01BX |
| Corning™ Costar™ Flat Bottom Cell Culture Plates (12-Well) | Corning | Cat# 07-200-82 |
| 70 μm cell strainer | Thermo Fisher Scientific | Cat# 76327 |
| Square coverslip | Thermo Fisher Scientific | Cat# 15-183-88 |
| 3P PVC tubing for perfusion 1.02 mm in diameter | Thermo Fisher Scientific | Cat# NC0279757 |
| Double-Ended Micro-Tapered Stainless-Steel Spatula | Thermo Fisher Scientific | Cat# 2140110 |
| Iris Scissors | VWR | Cat# 470018-890 |
| MeisterHand LISTER Bandage scissors 8" (20.3) | HOSPEQ | Cat# MH50550 |
| FisherbrandTM Standard Dissecting Scissors | Fisher Scientific | Cat# 0895120 |
| Medium Tip dissecting Forceps | VWR | Cat# 470001-846 |
| Premium Superfrost Microscope slides | Fisher Scientific | Cat# 12-544-7 |
| BD Hypodermic Needles, 27G | BD | Cat# 305110 |
| Kimwipes | Fisher Scientific | Cat# 06-666 |
| Embedding mold | Thermo Fisher Scientific | Cat# 50465347 |
| 3P Minipuls Peristaltic Pump | Fisher Scientific | Cat# NC0745002 |
| Leica CM3050S Cryostat | Leica BIOSYSTEMS | Cat# 958846 |
| Fisherbrand™ Slide Drying Bench | Fisher Scientific | Cat# 11-474-470 |
| Zeiss LSM710 | Zeiss | Cat# LSM-170 |
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