Reagent | Final concentration | Amount |
---|---|---|
Tris pH 8 (1 M) | 16.7 mM | 8.35 mL |
EDTA pH 8 (100 mM) | 1.2 mM | 6 mL |
NaCl (5 M) | 167 mM | 16.7 mL |
SDS (10%) | 0.1% | 5 mL |
Triton X100 | 1.84% | 9.2 mL |
ddH2O | n/a | 454.75 mL |
Total | n/a | 500 mL |
Store at 4°C for up to a year