Reagent | Final concentration | Amount |
---|---|---|
Tris pH 8 (1 M) | 100 mM | 50 mL |
LiCl | 500 mM | 10.6 g |
Sodium deoxycholate | 1% | 5 g |
NP-40 | 1% | 5 mL |
ddH2O | n/a | up to total |
Total | n/a | 50 mL |
Store at 18°C–25°C for up to a year
Reagent | Final concentration | Amount |
---|---|---|
Tris pH 8 (1 M) | 100 mM | 50 mL |
LiCl | 500 mM | 10.6 g |
Sodium deoxycholate | 1% | 5 g |
NP-40 | 1% | 5 mL |
ddH2O | n/a | up to total |
Total | n/a | 50 mL |
Store at 18°C–25°C for up to a year