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GFP-Rap2a and mCherry-Rab5 live dynamics. Live 2D time-lapse imaging of MDA-MB-231 cells stably expressing GFP-Rap2a, transiently transfected with mCherry-Rab5. 5-s intervals (plus exposure time), 100 frames. 5 fps. Nearest neighbor deconvolution. Widefield microscope. Scale bar, 5 µm. Note that GFP-Rap2a and mCherry-Rab5 do not exactly overlap in the endosomal structure, which is likely due to a delay in image capture because of exposure time.
